Principal Investigator

Picture of Steven Short in office in front of a window with a view of a UTM building in the background

Dr Steven M Short

I earned my Combined Honours BSc in Biology and Oceanography at the University of British Columbia in 1995. I obtained my PhD from UBC in 2002. After that, I worked at the Ocean Sciences Department at the University of California, Santa Cruz as a postdoctoral researcher. In 2004 I started my independent research program as an Assistant Professor in the Department of Biology at the University of Denver. In 2007, I joined the Department of Biology at the University of Toronto Mississauga an Associate Professor and the Department Chair. I am appointed as graduate faculty in the Department of Ecology and Evolutionary Biology, a tri-campus graduate unit at the University of Toronto, and the Department of Physical & Environmental Sciences at University of Toronto Scarborough. At UTM, I teach undergraduate classes in Microbial Ecology and Virology and supervise undergraduate and graduate research students.


Research Staff

  • Dr. Ichiro Inamoto, PhD.

    • Lab Manager, Isolation, purification, and transmission electron microscopy of Chrysochromulina parva viruses.

  • Dr. Christine Palermo, PhD.

    • Genomic analysis and bioinformatics of uncharcterized algal viruses infecting Chrysochromulina parva.


Graduate Students

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Karolina Szlapa

Department of Ecology and Evolutionary Biology, University of Toronto, PhD student.


Undergraduate Research Students

  • Daphne Arguelles

    • BIO481Y, Biology Research Project. Monitoring the weekly dynamics of algal viruses in two distinct freshwater environments in Ontario, Canada.

    • University of Toronto Excellence Award, Summer 2022. Development and validation of multiplex TaqMan assays to quantify algal viruses in mixed cultures.

    • BIO399Y, Research Opportunities Program: Purifying and characterizing viruses and virophages that coinfect the alga Chrysochromulina parva.

  • Simran Rakhra

    • BIO481Y, Biology Research Project. The diversity of prymnesiophyte algae (haptophyta) in Lake Ontario and a nearby urban pond.

  • Gurshan Bajaj

    • BIO399Y, Research Opportunities Program. Purifying and characterizing viruses co-infecting the alga Chrysochromulina parva through end-point dilutions and quantitative PCR.


Short Lab Alumni

Graduate Students

  • Dr. Christine Palermo, PhD 2022

    • Thesis: Diversity, Relative Abundance, and Functional Potential of Viral and Bacterial Communities. University of Toronto Scarborough, Department of Physical and Environmental Sciences.

  • Dr. Dylan Shea, PhD 2021

    • Thesis: The Influence of Aquaculture on Marine Microbiota and Pathogen Communities in a British Columbia Coastal Ecosystem. Co-supervised with Prof. Martin Krkosek, University of Toronto, Department of Ecology and Evolutionary Biology.

  • Dr. Samantha Cheung, PhD 2021

    • Thesis: Application of Molecular Tools to Determine the Effects of Key Growth Factors on Cultivated Algal Biofilm Communities. Co-supervisor with D. Grant Allen, University of Toronto, Department of Chemical Engineering and Applied Chemistry.

  • Rose Mastin Wood, MSc 2021

    • Thesis: Examination of Infection Dynamics within a Viral Consortium Infecting the Haptophyte Alga Chrysochromulina parva. University of Toronto, Department of Ecology and Evolutionary Biology.

  • Alex Paquette, MSc 2017

    • Thesis: Understanding the Microbial Community of a Microalgal Biofilm from a Photobioreactor. Co-supervisor with George Espie, University of Toronto, Department of Cell and Systems Biology.

  • Dr. Michael Staniewski, PhD 2017

    • Thesis: Grazer and Virus-Mediated Mortality of Freshwater Phytoplankton. University of Toronto, Department of Ecology and Evolutionary Biology.

  • Dr. Andrew Long, PhD 2017

    • Thesis: The Persistence of Algal Viruses in Freshwater Environments. University of Toronto, Department of Ecology and Evolutionary Biology.

  • Lindsey Ogston, MSc 2015

    • Thesis: The Prevalence of Fish Viruses Associated with Aquaculture Enclosures in B.C. Coastal Waters. Co-supervisor with Martin Krkosek, University of Toronto, Department of Ecology and Evolutionary Biology.

  • Robin Rozon, MSc 2012

    • Thesis: Algal virus dynamics and ecology in the Bay of Quinte, Canada. University of Toronto, Department of Ecology and Evolutionary Biology.

  • Oksana Rusanova, MSc 2010

    • Thesis: Seasonal Abundance of Chlorella Viruses in Two Contrasting Freshwater Environments of Ontario, Canada. University of Toronto, Department of Ecology and Evolutionary Biology.

  • Claire Olsofka, MSc 2008

    • Thesis: A Quantitative Molecular Technique for Studying Competition among Strains of Micromonas Pusilla. University of Denver, Department of Biological Sciences.

Undergraduate Research Students

  • Deryk Vu, HBSc 2021

    • TEM Visualization of Algal Viruses Purified by Gradient Ultracentrifugation (BIO481 2020/2021)

    • Isolating and studying components of a complex virus-host system (NSERC USRA 2020)

    • Understanding the nature of virophages, viruses that parasitize other viruses (BIO399 2019/2020)

  • Cynthia Ton, HBSc 2019

    • Research Assistant, Maintenance and growth of Chrysochromulina parva and its viruses.

  • Ajandan Nandakumar, HBSc 2020

    • Using the Rhodopsin Gene as a Novel Molecular Target for Effective Quantification of C. parva, an Important Phytoplankton (BIO481 2019/2020)

    • Examining the Dynamics of a Complex Virus-Host System (NSERC USRA 2019)

    • Evaluating the Distribution of Algae Infecting DNA Viruses in Coastal British Columbia (BIO399 2018/2019)

  • Jenna Black, HBSc 2019

    • Understanding the Complexity of a Novel Virophage-Virus-Host System (BIO481 2018/2019)

    • Understanding the Complexity of a Novel Virophage-Virus-Host System (NSERC USRA 2018)

    • A Complex Community of Viruses and Virophages Infects the Alga C. parva (BIO399 2017/2018)

  • Ammar Hafeez, HBSc 2018

    • Fine-Scale Temporal Dynamics of a Novel Algal Virus-Host System (BIO481 2017/2018)

    • Fine-Scale Temporal Dynamics of a Novel Algal Virus-Host System (NSERC USRA 2017)

  • Nikhil George, HBSc 2017

    • Spatial-Temporal Analyses of Algal Virus Genotypes in Hamilton Harbour (BIO481 2016/2017)

    • Spatial-Temporal Analyses of Algal Virus Diversity and Abundance Throughout Hamilton Harbour (BIO399 2015/2016)

  • Yuri Chaban, HBSc 2016

    • Investigating Host-Virus Interactions Between the Freshwater Algae Chrysochromulina parva and its Virus, CpV-BQ1, Using a Modified Dilution Assay (BIO481 2015/2016)

    • Clonal Variation of Viruses that Infect the Freshwater Haptophyte Chrysochromulina parva (NSERC USRA 2015)

    • Investigating the Diversity of Algae-Infecting Chloroviruses and the Effect of Nutrient Treatment on Primary Productivity in KSR Experimental Ponds (BIO399 2014/2015)

  • Amna Alam, HBSc 2015

    • Biogeography of Freshwater Algal Virus Communities: An Analysis of the Diversity in Lake Ontario, Lake Kinneret, and Lake Taihu (BIO481 2014/2015)

  • Samia Mirza, HBSc 2014

    • Isolation and Characterization of Viruses Infecting the Freshwater Algae Chrysochromulina parva (BIO481 2013/2014)

    • Virus Parasitism of Chrysochromulina parva, a Globally Important Freshwater Phytoplankton (NSERC USRA 2013)

    • Molecular Analysis of Phytoplankton and Their Viruses (BIO399 2012/2013).

  • Ankita Virdi, HBSc 2013

    • The Biodiversity of Algal Viruses in the Great Lakes (BIO481 2012/2013)

    • Cultivation and Molecular Characterization of Freshwater Phytoplankton (NSERC USRA 2012)

  • Rawana Hayour, BSc 2012

    • Which Marker Gene is Better to Study Phytoplankton Diversity and Taxonomic Resolution: psbA or 18S rDNA? (BIO481 2011/2012)

  • Michael Lagowski, HBSc 2012

    • Phylogenetic Analysis of the DNA Polymerase (polB) Gene Reveals Phycodnaviridae Diversity Within and Among the Great Lakes (BIO481 2011/2012)

  • Saba Haroon, HBSc 2012

    • Molecular Identification of Phytoplankton Isolated from Ontario Freshwaters (BIO399 2010/2011)

  • Michael Staniewski, HBSc 2010

    • Modified Dilution Method to Estimate Grazing and Virus-Induced Mortality of Phytoplankton in the UTM Storm Water Management Pond (BIO481 2009/2010)

  • Natasha Musrap, HBSc 2010

    • Seasonal Abundances of Chlorella Species in Ontario Freshwater Environments (BIO481 2009/2010)

    • Molecular Analysis of Freshwater Microorganisms (BIO399 2008/2009)

  • Oksana Rusanova, HBSc 2009

    • Spatial and Temporal Analysis of Lake Ontario Phycodnavirus Communities (BIO481 2008/2009)